Puerto Maldonado is a city in Southeastern Peru in the Amazon rainforest 55 kilometres (34 mi) west of the Bolivian border; located at the confluence of the Tambopata and Madre de Dios rivers, the latter of which joins the Madeira River as a tributary of the Amazon. It is the capital of the Madre de Dios Region.

Nearby are the Manú National Park, Tambopata National Reserve, and Bahuaja-Sonene National Park, which have been established to protect natural resources. These are some of the most pristine primary rain forests in the world. They include several oxbow lakes and clay licks, where hundreds of birds, including macaws, feed on clay.

Puerto Maldonado is in the tropical Amazon Basin. The climate is hot and humid at all times. The average annual temperature is 26 °C (79 °F) with the months of August and September being the hottest. Annual rainfall exceeds 1,000 millimetres (3.3 ft). The wet season is from October to April. The main part of the town is located on a slightly elevated area that does not normally flood in the wet season. Road travel often becomes impossible during this time. A low season occurs between June and August.   common phenomenon known locally as a surazo or friaje occurs when polar winds blow in from the mountainous south. The temperature drops to as low as 8 °C (46 °F) for several days.